ImmunoCAP® ISAC and Microtest for multiplex allergen testing in people with difficult to manage allergic disease: a systematic review and cost analysis. A combination of innovative biochip technology with cutting edge research in molecular allergology has resulted in ImmunoCAP® ISAC – the most advanced in. The immuno-solid phase allergen chip (ISAC) test (Thermo Fisher ImmunoCAP ISAC) is a microarray assay system designed to assay specific.
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Inclusion and exclusion criteria Population Adults and children with difficult to manage allergic disease who are being assessed in secondary or tertiary care settings.
See Stability for additional info.
ImmunoCAP ISAC is the first multiplex in vitro diagnostic tool for the allergy specialist that is based exclusively on allergen components. Imkunocap general, individual ISAC components tended to have high specificity, but low sensitivity, relative to whole-allergen single IgE tests or SPT for the prediction of allergic response. We did not identify any studies of Microtest which met the inclusion criteria for this review. Preferred temperatures for storage prior to and during shipping to ARUP.
Two studies investigated the effect on diagnostic classification of immunocao ImmunoCAP ISAC testing to the standard diagnostic work-up of people with allergic disease. All potentially relevant studies cited in documents supplied by the test manufacturers had already been identified by bibliographic database searches. This issue may be freely reproduced for the purposes of isad research and study and extracts or indeed, the full report may be included in professional journals provided that suitable acknowledgement is made and the reproduction is not associated with any isad of advertising.
See Appendix 1 for all search strategies. Chapter 3, Assessment of clinical effectiveness. SPT, OFC, single IgE ; details of the reference standard test diagnostic accuracy studies only ; outcome measures included change to treatment or treatment plan, e.
Immuunocap Technology Assessment, No. The diagnostic accuracy of ISACwhen used to measure three individual egg components Gal d1 or Gal d2 or Gal d3was compared with the accuracy of single IgE tests egg yolk or egg white and compared with the accuracy of SPTs egg white extract or raw egg white or boiled egg white or egg yolk extract or raw egg yolk or boiled egg yolk.
One study 47 was reported only as a conference abstract. Based on the searches and inclusion screening described above see Search strategy and Inclusion and exclusion criteriaabove20 publications, 30 — 49 of 15 studies, were included in the review; the results section of this report cites studies using the primary publication and, where this is different, the publication in which the referenced data were reported.
The results of the only study to investigate serial testing suggested that use of ImmunoCAP ISAC after single IgE testing only in participants who were negative on single IgE could increase sensitivity relative to single IgE alone without any loss in specificity.
Allergen Panel, IgE by ImmunoCap ISAC
This enables patient investigations that require less time and resources. As was the case for studies of change to immjnocap, treatment or diagnosis, studies that did not specify that they included participants with difficult to manage allergic disease or describe inclusion criteria that could be considered consistent with this classification e.
The main potential sources of bias were in relation to participant selection and application of the index test. Further details of the excluded full papers and the reasons for exclusion can be found in Appendix 4. Multiplex allergen tests are molecular diagnostic tests, in the form of a glass slide, which can simultaneously test for the presence of multiple antibodies in blood samples up to 51 allergen sources.
ISAC Test: 112 Allergy Tests in one tiny blood sample!
The methods and assumptions used were largely unclear and the credibility of the assessments was questionable; therefore, jsac findings should be interpreted with extreme caution. These scoping searches were used to generate test sets of target references, which informed text mining analysis of high-frequency subject indexing terms using EndNote X7 reference management software Thomson Reuters, CA, USA.
Confidential information has been removed Johannes Savolainen, personal communication. To isav the effects on treatment e.
Oxford University Press; Cut-off values were not reported for the ISAC test. A systematic review was conducted to summarise the evidence on the clinical effectiveness and cost-effectiveness of ImmunoCAP ISAC and Microtest for multiplex allergen testing in people with allergic disease.
It is the result of a combination of innovative biochip technology and cutting edge research in molecular allergology. If you live at lsac distance from London, or for any reason cannot attend a face-to face appointment, please start with filling in an ISAC allergy symptoms questionnaire, which will be reviewed by one of our allergy consultants.
All presentations of allergic disease respiratory, skin, gastrointestinal, anaphylaxis were eligible for inclusion. For more information, visit our browser page. Results of the assessment inmunocap clinical effectiveness assessment The searches of bibliographic databases and conference abstracts identified references. Four economic analyses and 14 health-related quality of life studies were included ijmunocap the literature review.
The relatively low sensitivities of individual ISAC components are likely to be indicative of the proportions of patients in whom each component is associated with the observed allergic response. This tool has been designed to focus on elements of study design that we considered relevant to this specific study type, and is based upon the structure of the QUADAS-2 tool.
None of the eight studies 41 — 4749 reported the inclusion of patients with difficult to diagnose and manage allergic disease, or described inclusion criteria that could be considered consistent with this classification e. Fifteen studies were included in the review. Overview of included studies Based on the searches and inclusion screening described above see Search strategy and Inclusion and exclusion criteriaabove20 publications, 30 — 49 of 15 studies, were included in the review; the results section of this report cites studies using the primary publication and, where this is different, the publication in which the referenced data were reported.
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